Elsevier S & T

Ribonucleases, Part B: Artificial and Engineered Ribonucleases and Speicifc Applications: Artificial and Engineered Ribonucleases and Speicifc Applications

Nicholson, Allen W.

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Table of contents
  • Title pageiii
  • Copyright Pageiv
  • Table of Contentsv
  • Contributors to Volume 342ix
  • Prefacexv
  • Volume in Seriesxvii
  • Section I: Processing and Degradative Endoribonucleases1
  • Chapter 1. Purification and Activity Assays of the Catalytic Domains of the Kinase/Endoribonuclease3
  • Chapter 2. Monitoring Activation of Ribonuclease L by 2΄, 5΄-Oligoadenylates Using Purified Recomb10
  • Chapter 3. Accelerating RNA Decay through Intervention of RNase L: Alternative Synthesis of Composit20
  • Chapter 4. Polysomal Ribonuclease 128
  • Chapter 5. Liver Perchloric Acid-Soluble Ribonuclease44
  • Chapter 6. Escherichia coli Ribonuclease G55
  • Chapter 7. Escherichia coli Transcript Cleavage Factors GreA and GreB: Functions and Mechanisms of A64
  • A. Ribonucleoprotein Ribonucleases77
  • Chapter 8. Escherichia coli Ribonuclease P77
  • Chapter 9. Human Ribonuclease P93
  • Chapter 10. Saccharomyces cerevisiae Nuclear Ribonuclease P: Structure and Function101
  • Chapter 11. Cyanelle Ribonuclease P: Isolation and Structure–Function Studies of an Organellar Rib118
  • Chapter 12. Characterization of Ribonuclease MRP Function135
  • B. Double-Strand-Specific Ribonucleases135
  • Chapter 13. Escherichia coli Ribonuclease III: Affinity Purification of Hexahistidine-Tagged Enzyme143
  • Chapter 14. Purification and Characterization of Saccharomyces cerevisiae Rnt1p Nuclease159
  • Chapter 15. Pac1 Ribonuclease of Schizosaccharomyces pombe168
  • Chapter 16. Dictyostelium Double-Stranded Ribonuclease193
  • Chapter 17. Double-Stranded RNA Nuclease Associated with Rye Germ Ribosomes212
  • C. Ribonucleases That Cleave Atypical Phosphodiesters226
  • Chapter 18. Yeast mRNA Decapping Enzyme226
  • Chapter 19. RNA Lariat Debranching Enzyme233
  • Section II: Processing and Degradative Exoribonucleases249
  • A. 5΄–>3΄-Exoribonuclease251
  • Chapter 20. 5΄-Exoribonuclease 1: Xrn1251
  • Chapter 21. Rat1p Nuclease260
  • Chapter 22. Analysis of XRN Orthologs by Complementation of Yeast Mutants and Localization of XRN–269
  • Chapter 23. 5΄–>3΄-Exoribonuclease from Rabbit Reticulocytes282
  • Chapter 24. Drosophila 5΄–>3΄-Exoribonuclease Pacman293
  • B. 3΄–>5΄-Exoribonuclease293
  • Chapter 25. Purification of Poly(A)-Specific Ribonuclease303
  • Chapter 26. Escherichia coli Ribonuclease II309
  • Section III: Ribonuclease Complexes331
  • Chapter 27. Escherichia coli RNA Degradosome333
  • Chapter 28. Preparation of Escherichia coli Rne Protein and Reconstitution of RNA Degradosome346
  • Chapter 29. Purification of Yeast Exosome356
  • Section IV: Organellar Ribonucleases365
  • Chapter 30. Genetic and Biochemical Approaches for Analysis of Mitochondrial Degradosome from Saccha367
  • Chapter 31. Direct Sizing of RNA Fragments Using RNase- Generated Standards378
  • Chapter 32. Chlamydomonas reinhardtii as a Model System for Dissecting Chloroplast RNA Processing an384
  • Chapter 33. Chloroplast mRNA 3΄-End Nuclease Complex408
  • Chapter 34. Chloroplast p54 Endoribonuclease420
  • Section V: Viral Ribonucleases429
  • Chapter 35. Erns Protein of Pestiviruses431
  • Chapter 36. Herpes Simplex Virus vhs Protein440
  • Chapter 37. Influenza Virus Endoribonuclease451
  • Chapter 38. Bacteriophage T4 RegB Endoribonuclease467
  • Author Index481
  • Subject Index505
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822439
  • ISBN-13 9780080522579
  • Author Nicholson, Allen W.
  • Category Medical
  • Subject Biochemistry

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This second volume on ribonucleases provides up-to-date, methods-related information on these enzymes. Of particular interest to researchers will be the discussion of artificial and engineered ribonucleases, as well as the application of ribonucleases in medicine and biotechnology.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.