Elsevier S & T

RNA-Ligand Interactions, Part B: Molecular Biology Methods: Molecular Biology Methods

Simon, Melvin I.

In stock
No reviews
77.750 KD inc. VAT
License
Table of contents
  • Table of Contentsv
  • Contributors to Volume 318ix
  • Prefacexiii
  • Volumes in Seriesxv
  • Section I: Solution Probe Methods1
  • Chapter 1. Probing RNA Structure and RNA–Ligand Complexes with Chemical Probes3
  • Chapter 2. Methylene Blue-Mediated Cross-Linking of Proteins to Double-Stranded RNA22
  • Chapter 3. Structure and Distance Determination in RNA with Copper Phenanthroline Probing33
  • Chapter 4. Applications of Uranyl Cleavage Mapping of RNA Structure43
  • Chapter 5. Identification of Messenger RNAs That Contain Inosine48
  • Section II: Tethered-Probe Methodologies69
  • A. Photochemical Reagents69
  • Chapter 6. Site-Specific 4-Thiouridine Incorporation into RNA Molecules71
  • Chapter 7. Nucleoprotein Photo-Cross-Linking Using Halo- pyrimidine-Substituted RNAs88
  • Chapter 8. Structure Determination by Directed Photo-Cross- Linking in Large RNA Molecules with Site104
  • Chapter 9. Photolabile Derivatives of Oligonucleotides as Probes of Ribosomal Structure118
  • Chapter 10. Photoaffinity Cross-Linking and RNA Structure Analysis136
  • B. Chemical Reagents69
  • Chapter 11. Cleavage of RNA with Synthetic Ribonuclease Mimics147
  • Chapter 12. Site-Specific Sulfhydryl Groups for Study of RNA Conformation via Disulfide Cross-Linkin165
  • Chapter 13. Directed Hydroxyl Radical Probing Using Iron(II) Tethered to RNA175
  • Section III: In Vitro Affinity Selection Methodologies191
  • Chapter 14. In Vitro Selection of RNA Aptamers193
  • Chapter 15. In Vitro Selection and Characterization of RNAs with High Affinity to Antibiotics214
  • Chapter 16. Selection for RNA: Peptide Recognition through Sulfur Alkylation Chemistry229
  • Chapter 17. In Vitro Selection of RNA Substrates for Ribonuclease P and Its Catalytic RNA238
  • Chapter 18. RNA–Protein Interactions in Ribosomes. In Vitro Selection from Randomly Fragmented rRN251
  • Chapter 19. Optimized Synthesis of RNA–Protein Fusions for in Vitro Protein Selection268
  • Section IV: Genetic Methodologies for Detecting RNA–Protein Interactions295
  • A. Bacterial Systems295
  • Chapter 20. Screening RNA–Binding Libraries by Transcriptional Antitermination in Bacteria297
  • Chapter 21. Rapid Genetic Analysis of RNA–Protein Interactions by Translational Repression in Esch309
  • Chapter 22. RNA Challenge Phages as Genetic Tools for Study of RNA–Ligand Interactions332
  • B. Eukaryotic Systems295
  • Chapter 23. Screening RNA-Binding Libraries Using Tat-Fusion System in Mammalian Cells350
  • Chapter 24. Frameshifting Assay to Characterize RNA–Protein Interactions in Eukaryotic Cells363
  • Chapter 25. Translational Repression Assay Procedure. A Method to Study RNA–Protein Interactions i374
  • Chapter 26. Analysis of Low-Abundance Ribonucleoprotein Particles from Yeast by Affinity Chromatogra385
  • Chapter 27. Yeast Three-Hybrid System to Detect and Analyze RNA–Protein Interactions399
  • Section V: Protein Engineering Methodologies Useful for RNA–Protein Interaction Studies421
  • Chapter 28. Using Peptides as Models of RNA–Protein Interactions423
  • Chapter 29. Analysis of RNA–Protein Cross-Link Sites by BERND THIEDE AND Matrix-Assisted Laser Des438
  • Chapter 30. In Vitro Reconstitution of 30S Ribosomal Subunits Using Complete Set of Recombinant Prot446
  • Chapter 31. Directed Hydroxyl Radical Probing of RNA from Iron(II) Tethered to Proteins in Ribonucle461
  • Section VI: Cell Biology Methods477
  • Chapter 32. Use of Dimethyl Sulfate to Probe RNA Structure in Vivo479
  • Chapter 33. Sensitive and High-Resolution Detection of RNA in Situ493
  • Author Index507
  • Subject Index525
  • Color Plate SectionColor Plate-1
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822194
  • ISBN-13 9780080496757
  • Author Simon, Melvin I.
  • Category Science
  • Subject Biochemistry

Do you have questions about this book?

Ask an expert!

Description eBook delivery & access Reviews
RNA-Ligand Interactions, Part B focuses on molecular biology methods. Major topics covered include: solution probe methods, tethered-probe methodologies, in vitro affinity selection methodologies, genetic methodologies for detecting RNA-protein interactions, protein engineering methodologies useful for RNA-protein interaction studies, and cell biology methods.
RNA-Ligand Interactions, Part A, its companion, VOLUME 317 focuses on structural biology methods.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.