Elsevier S & T

RNA Polymerase and Associated Factors, Part D

Adhya, Sankar; Garges, Susan

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Table of contents

Title Pageiii
Copyright Pageiv
Table of Contentsv
Contributors to Volume 371xi
Volumes in Seriesxix
Section I: Transcription Elongation1
Chapter 1. On Models and Methods for Studying Polymerase Translocation3
Chapter 2. Evaluation of Fluorescence Spectroscopy Methods for Mapping Melted Regions of DNA Along t13
Chapter 3. Single DNA Molecule Analysis of Transcription Complexes34
Chapter 4. Assay for Movements of RNA Polymerase along DNA50
Chapter 5. Kinetics of Multisubunit RNA Polymerases: Experimental Methods and Data Analysis71
Chapter 6. Principles and Methods of Affinity Cleavage in Studying Transcription82
Chapter 7. Iodine-125 Radioprobing of E. coli RNA Polymerase Transcription Elongation Complexes106
Chapter 8. Formation of Long DNA Templates Containing Site-Specific Alkane–Disulfide DNA Interstra120
Chapter 9. Probing the Organization of Transcription Complexes Using Photoreactive 4-Thio- Substitut133
Chapter 10. Fluorescence Resonance Energy Transfer (FRET) in Analysis of Transcription- Complex Stru144
Chapter 11. Methods of Walking with the RNA Polymerase160
Chapter 12. Stepwise Walking and Cross-Linking of RNA with Elongating T7 RNA Polymerase170
Chapter 13. Characterization of Protein–Nucleic Acid Interactions that are Required for Transcript179
Chapter 14. Strategies and Methods of Cross-Linking of RNA Polymerase Active Center191
Chapter 15. Using a lac Repressor Roadblock to Analyze the E. Coli Transcription Elongation Complex207
Chapter 16. Biochemical Assays of Gre Factors of Thermus Thermophilus219
Chapter 17. Engineering of Elongation Complexes of Bacterial and Yeast RNA Polymerases233
Chapter 18. Assay of Transient State Kinetics of RNA Polymerase II Elongation252
Chapter 19. Analysis of RNA Polymerase II Elongation In vitro264
Chapter 20. Preparation and Assay of RNA Polymerase II Elongation Factors Elongin and ELL276
Chapter 21. Use Of RNA Yeast Polymerase II Mutants in Studying Transcription Elongation284
Chapter 22. Acetylation of Human AP-Endonuclease 1, A Critical Enzyme in DNA Repair and Transcriptio292
Chapter 23. Characterization of Transcription-Repair Coupling Factors in E. Coli and Humans300
Chapter 24. Techniques to Analyze the HIV-1 Tat and TAR RNA-Dependent Recruitment and Activation of324
Section II: Transcription Termination337
Chapter 25. Assay of Intrinsic Transcription Termination by E. Coli RNA Polymerase on Single-Strande339
Chapter 26. Bacteriophage HK022 Nun Protein: A Specific Transcription Termination Factor that Exclud351
Chapter 27. Assay of Transcription Termination by Ribosomal Protein L4356
Chapter 28. Analysis of the Intrinsic Transcription Termination Mechanism and Its Control369
Chapter 29. Rho’s Role in Transcription Attenuation in the tna Operon of E. Coli383
Chapter 30. Role of RNA Structure in Transcription Attenuation in Bacillus subtilis: The trpEDCFBA O392
Chapter 31. TRAP–RNA Interactions Involved in Regulating Transcription Attenuation of the Bacillus405
Chapter 32. Analyzing Transcription Antitermination in Lambdoid Phages Encoding Toxin Genes418
Chapter 33. Genetic and Biochemical Strategies to Elucidate the Architecture and Targets of a Proces438
Chapter 34. Assay of Transcription Antitermination by Proteins of the CspA Family460
Chapter 35. Assay of Antitermination of Ribosomal RNA Transcription472
Section III: Chromatin489
Chapter 36. Purification of Elongating RNA Polymerase II and Other Factors from Yeast Chromatin491
Chapter 37. Chromatin Assembly In Vitro with Purified Recombinant ACF and NAP-1499
Chapter 38. Tying C’ Ends of H2A and H2B Using a Molecular Glue, Tissue-Type Transglutaminase516
Chapter 39. Chromatin Decompaction Method by HMGN Proteins521
Chapter 40. Assay of Activator Recruitment of Chromatin- Modifying Complexes536
Chapter 41. Insights into Structure and Function of GCN5/ PCAF and yEsa 1 Histone Acetyltransferase545
Chapter 42. Assay of the Fate of the Nucleosome During Transcription by RNA Polymerase II564
Chapter 43. Probing Chromatin Immunoprecipitates with CpG-Island Microarrays to Identify Genomic Sit577
Chapter 44. Isolation of RNA Polymerase Suppressors of a (p)ppGpp Deficiency596
Chapter 45. Analysis of Transcriptional Repression by Mig1 in Saccharomyces cerevisiae Using a Repor602
Chapter 46. Mutational Analysis of Drosphila RNA Polymerase II615
Author Index631
Subject Index659

Book details

Vendor Elsevier S & T
SKU 9780121822743
ISBN-13 9780080522609
Author Adhya, Sankar; Garges, Susan
Category Science
Subject Biochemistry

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RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein.

* Models and methods for studying polymerase translocation
* Assay for movements of RNA polymerase along DNA
* Engineering of elongation complexes of bacterial and yeast RNA polymerases

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