Elsevier S & T

Sphingolipid Metabolism and Cell Signaling, Part B

Abelson, John N.; Simon, Melvin I.

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Table of contents
  • Table of Contentsv
  • Contributors to Volume 312xi
  • Prefacexvii
  • Volumes in Seriesxix
  • Section I: Methods for Analyzing Sphingolipids1
  • Chapter 1. Analysis of Sphingoid Bases and Sphingoid Base 1-Phosphates by High-Performance Liquid Ch3
  • Chapter 2. Enzymatic Method for Measurement of Sphingosine 1-Phosphate9
  • Chapter 3. Ceramide Mass Analysis by Normal-Phase High-Performance Liquid Chromatography16
  • Chapter 4. Quantitative Determination of Ceramide Using Diglyceride Kinase22
  • Chapter 5. Analysis of Sphingomyelin, Glucosylceramide, Ceramide, Sphingosine, and Sphingosine 1-Pho32
  • Chapter 6. Analyses of Glycosphingolipids by High-Performance Liquid Chromatography45
  • Chapter 7. Sphingolipid Extraction and Analysis by Thin-Layer Chromatography64
  • Chapter 8. Extraction and Analysis of Multiple Sphingolipids from a Single Sample80
  • Chapter 9. Purification of Sphingolipid Classes by Solid-Phase Extraction with Aminopropyl and Weak101
  • Chapter 10. Ganglioside Analysis by High-Performance Thin-Layer Chromatography115
  • Chapter 11. Purification and Analysis of Gangliosides135
  • Chapter 12. Thin-Layer Chromatography Blotting Using Polyvinylidene Difluoride Membrane (Far-Eastern145
  • Chapter 13. Thin-Layer Chromatography Immunostaining157
  • Chapter 14. Monoclonal Anti-Glycosphingolipid Antibodies160
  • Chapter 15. Immunolocalization of Gangliosides by Light Microscopy Using Anti-Ganglioside Antibodies179
  • Chapter 16. Cloud-Point Extraction of Gangliosides Using Nonionic Detergent C14EO6187
  • Chapter 17. Analyses of Glycosphingolipids Using Clam, Mercenaria mercenaria, Ceramide Glycanase196
  • Chapter 18. Quantitative Analyses of Binding Affinity and Specificity for Glycolipid Receptors by Su205
  • Chapter 19. Use of Circular Dichroism for Assigning Stereochemistry of Sphingosine and Other Long-Ch217
  • Chapter 20. Infrared Determination of Conformational Order and Phase Behavior in Ceramides and Strat228
  • Chapter 21. Use of Nuclear Magnetic Resonance Spectroscopy in Evaluation of Ganglioside Structure, C247
  • Chapter 22. Fluorescence Quenching Assay of Sphingolipid/ Phospholipid Phase Separation in Model Mem272
  • Section II: Methods for Analyzing Aspects of Sphingolipid Metabolism in Intact Cells291
  • Chapter 23. Synthesis of Fluorescent Substrates and Their Application to Study of Sphingolipid Metab293
  • Chapter 24. Selection of Mammalian Cell Mutants in Sphingolipid Biosynthesis304
  • Chapter 25. Selection of Yeast Mutants in Sphingolipid Metabolism317
  • Chapter 26. Fluorescence-Based Selection of Gene-Corrected Hematopoietic Stem and Progenitor Cells B330
  • Chapter 27. Mammalian Ganglioside Sialidases: Preparation and Activity Assays339
  • Section III: Sphingolipid–Protein Interactions and Cellular Targets359
  • Chapter 28. Effects of Sphingosine and Other Sphingolipids on Protein Kinase C361
  • Chapter 29. Kinetic Analysis of Sphingoid Base Inhibition of Yeast Phosphatidate Phosphatase373
  • Chapter 30. Assays of Sphingosine-Dependent Kinase for 14-3-3 Protein381
  • Chapter 31. Synthesis and Use of Caged Sphingolipids387
  • Chapter 32. Binding of Sphingosine 1-Phosphate to Cell Surface Receptors401
  • Chapter 33. Use of Short-Chain Ceramides407
  • Chapter 34. Analysis of Ceramide-Activated Protein Phosphatases420
  • Chapter 35. Use of Affinity Chromatography and TID-Ceramide Photoaffinity Labeling for Detection of429
  • Chapter 36. Lectin-Mediated Cell Adhesion to Immobilized Glycosphingolipids438
  • Chapter 37. Analysis of Glycolipid-Dependent Cell Adhesion Based on Carbohydrate–Carbohydrate Inte447
  • Chapter 38. Analysis of Interactions between Glycosphingolipids and Microbial Toxins459
  • Chapter 39. Oxidation of Aglycone of Glycosphingolipids: Serine and Ceramide Acid Precursors for Sol473
  • Chapter 40. Separation of Glycosphingolipid-Enriched Microdomains from Caveolae Characterized by Pre488
  • Chapter 41. Reconstitution of Sphingolipid–Cholesterol Plasma Membrane Microdomains for Studies of495
  • Chapter 42. Analysis of Ceramides Present in Glycosylphosphatidylinositol Anchored Proteins of Sacch506
  • Chapter 43. Preparation of Functionalized Lipid Tubules for Electron Crystallography of Macromolecul515
  • Section IV: Sphingolipid Transport and Trafficking521
  • Chapter 44. Applications of BODIPY–Sphingolipid Analogs to Study Lipid Traffic and Metabolism in C523
  • Chapter 45. Using Biotinylated Gangliosides to Study Their Distribution and Traffic in Cells by Immu534
  • Chapter 46. Assays for Transmembrane Movement of Sphingolipids562
  • Section V: Other Methods581
  • Chapter 47. Compilation of Methods Published in Previous Volumes of Methods in Enzymology583
  • Author Index593
  • Subject Index631
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822132
  • ISBN-13 9780080496696
  • Author Abelson, John N.; Simon, Melvin I.
  • Category Science
  • Subject Biochemistry

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This volume contains information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets. Its companion Volume 311 presents methods used in studying enzymes of sphingolipid biosynthesis and turnover, including inhibitors of some of these enzymes, genetic approaches, and organic and enzymatic syntheses of sphingolipids and analogs.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.