Stem Cell Culture: Methods in Cell Biology
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Table of contents
- Contentsv
- Contributorsxi
- Prefacexv
- Biographyxix
- Chapter 1: Derivation of Human Embryonic Stem Cells in Standard and Chemically Defined Conditions1
- I. Introduction1
- II. Methods2
- III. Discussion12
- IV. Materials12
- References13
- Chapter 2: Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic Stem Cells in Feed15
- I. Introduction
- II. Methods and Materials17
- III. Results21
- IV. Discussion and Summary
- References28
- Chapter 3: Microporous Membrane Growth Substrates for Embryonic Stem Cell Culture and Differentiatio29
- I. Introduction
- II. Rationale32
- III. Methods
- IV. Summary
- References55
- Chapter 4: Culture of Rodent Spermatogonial Stem Cells, Male Germline Stem Cells of the Postnatal An59
- I. Introduction60
- II. Rationale63
- III. Methods67
- IV. Materials78
- V. Discussion80
- References81
- Chapter 5: Characterization of Human Amniotic Fluid Stem Cells and Their Pluripotential Capability85
- I. Introduction86
- II. Amniotic Fluid Cell Composition and Stem Cells87
- III. In Vitro Potential of Amniotic Fluid Stem Cells91
- IV. Ex Vivo and In Vivo Application of Amniotic Fluid Stem Cells94
- V. Discussion97
- References98
- Chapter 6: Method to Isolate Mesenchymal-Like Cells from Wharton's Jelly of Umbilical Cord101
- I. Introduction102
- II. Rationale103
- III. Methods103
- IV. Materials110
- V. UCMSCs and Culture Characteristics115
- VI. Discussion116
- References118
- Chapter 7: Isolation, Characterization, and Differentiation of Human Umbilical Cord Perivascular Cel121
- I. Introduction122
- II. Methods123
- III. Concluding Remarks133
- References134
- Chapter 8: Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance137
- I. Introduction139
- II. Epithelial-Mesenchymal Relationship139
- III. Stem Cells and Lineage Biology140
- IV. Liver Processing150
- V. Fractionation of Liver Cell Subpopulations162
- VI. Feeder Cells170
- VII. Extracellular Matrix174
- VIII. Culture Media176
- IX. Monolayer Cultures of Cells180
- X. Three-Dimensional Systems185
- Appendix 1: Nomenclature, Glossary, and Abbreviations195
- Appendix 2: Details on the Preparation of Buffers/Reagents207
- Appendix 3: Commercial Sources of Reagents211
- Appendix 4: Sources of Primary and Secondary Antibodies for Immunoselection and for Characterization212
- References213
- Chapter 9: Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo227
- I. Introduction228
- II. Rationale229
- III. Methods229
- IV. Materials237
- V. Discussion237
- VI. Summary238
- References239
- Chapter 10: Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells241
- I. Introduction242
- II. Rationale243
- III. Methods245
- IV. Materials250
- V. Discussion253
- VI. Summary254
- References254
- Chapter 11: Isolation of Human Mesenchymal Stem Cells from Bone and Adipose Tissue257
- I. Introduction258
- II. Isolation of hMSCs from Trabecular Bone259
- III. Isolation of hMSCs from Adipose Tissue263
- IV. Growth, Subculture, and Cryopreservation of Undifferentiated hMSCs265
- V. Surface Marker Characterization of hMSCs267
- VI. hMSC Differentiation Assays for Osteogenic, Adipogenic, and Chondrogenic Pathways270
- VII. Summary276
- References276
- Chapter 12: Culture of Mesenchymal Stem/Progenitor Cells in Adhesion-Independent Conditions279
- I. Introduction280
- II. Suspension Culture of Bone Marrow-Derived MPCs280
- III. Analysis of Suspension Culture283
- IV. Mechanism of MPC Expansion in Suspension288
- V. Discussion291
- References292
- Chapter 13: Purification and Long-Term Culture of Multipotent Progenitor Cells Affiliated with the W295
- I. Introduction297
- II. Materials and Methods: Cell Analysis and Purification by Flow Cytometry298
- III. Materials and Methods: Long-Term Culture of Human Blood Vessel-Associated Progenitor Cells301
- IV. Materials and Methods: Proliferation Kinetics of Human Myoendothelial Cells and Pericytes303
- V. Materials and Methods: Genotypic and Phenotypic Analyses of Long-Term Cultured Cells304
- VI. Conclusion306
- References308
- Chapter 14: Isolation and Culture of Colon Cancer Stem Cells311
- I. Introduction312
- II. Identification of CSCs in Colon Cancer Tissue314
- III. Isolation and Propagation of Colon CSCs315
- IV. Discussion322
- References323
- Chapter 15: Isolation and Establishment of Human Tumor Stem Cells325
- I. Introduction326
- II. Rationale327
- III. Methods327
- IV. Results336
- V. Discussion338
- VI. Materials340
- References341
- Chapter 16: Stem Cells from Cartilaginous and Bony Fish343
- I. Introduction to Models and Uses344
- II. Embryonal Stem Cells348
- III. Tissue-Specific Stem Cells353
- IV. Outlook and Future Contributions361
- References362
- Index369
- Volumes In Series379
The purpose of this volume is to provide a comprehensive resource for researchers in the fields of embyonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentitation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Authors will focus on the practical do's and don'ts of isolating and culturing these cell types, and feel free to use illustrative data or diagrams whereever this improves the comprehension of the reader. This should allow the reader to compare and contrast techniques and make this a standard reference for those in the field, or desiring to start stem cell culture.
* Describes techniques in stem cell research
* Delineates critical steps and potential pitfalls for each method
*covers specific procedures in dealing with Human Embryonic Stem Cells
* Describes techniques in stem cell research
* Delineates critical steps and potential pitfalls for each method
*covers specific procedures in dealing with Human Embryonic Stem Cells