Elsevier S & T

The SV40 Replicon Model for Analysis of Anticancer Drugs

Snapka, Robert M.

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Table of contents
  • Cover
  • Copyright Pageii
  • Contentsv
  • Chapter 1. Papovavirus Models for Mammalian Replicons1
  • Papovaviruses2
  • The SV40 Genome3
  • SV40 Chromatin3
  • Chapter 2. Analysis of SV40 DNA Replication Intermediates15
  • Extraction of Viral DNA and Chromatin15
  • Radiolabeling of SV40 DNA16
  • Sucrose Density Gradient Ultracentrifugation17
  • Isopycnic Centrifugation18
  • High-Resolution Electrophoresis19
  • Electron Microscopy32
  • Filter Assays34
  • Chapter 3. Catastrophic Failure of DNA Replication Forks: Structural and Recombinational Pathways39
  • Collision of DNA Replication Forks with Drug-Stabilized Topoisomerase I-DNA Strand Passing Complexes43
  • DNA Polymerase Inhibition53
  • SV40 Replication Fork Instability and Mammalian Replicons61
  • Chapter 4. Topoisomerase II and Termination of DNA Replication65
  • The Termination Problem65
  • Catalytic Topoisomerase II Inhibitors and Topoisomerase II Poisons76
  • Assays for Catenated Dimers83
  • Chapter 5. DNA Damaging Agents101
  • Reactive Aldehydes102
  • Ultraviolet (UV) Radiation105
  • DNA Alkylating Agents109
  • Chapter 6. Drug Discovery and Evaluation with SV40127
  • Topoisomerase I Poison Signature Pattern129
  • Topoisomerase II Inhibition and Poisoning129
  • Inhibition of Protein Synthesis131
  • DNA Replication Fork Arrest132
  • Protein-DNA Crosslinkers133
  • DNA Alkylating Agents133
  • Validation of Assay133
  • Detection and Purification of Topoisomerase II Inhibitors Using the SV40 Assay137
  • Chapter 7. SV40 In Vitro DNA Replication: A Potential Model For Studying Anticancer Drug Action149
  • Viral Components Required for SV40 In Vitro DNA Replication150
  • Mammalian Cell Proteins Required for the Efficient Replication of SV40 Origin Containing DNA In Vitr151
  • A Multiprotein Form of DNA Polymerase Mediates Papovavirus DNA Replication In Vitro154
  • Anticancer Drug Action on In Vitro SV40 DNA Replication Activity in Crude Cell Extracts155
  • Anticancer Drug Action on In Vitro SV40 DNA Synthesis Mediated by a Multiprotein Form ofDNA Polymera157
  • Perspective158
  • Chapter 8. SV40 Circular Oligomer Series: Normal and Abnormal DNA Replication Intermediates167
  • The Order of SV40 Circular Oligomers170
  • Normal and Aberrant Replication Intermediates of the SV40 Circular Oligomers177
  • Index187
Book details
  • Vendor Elsevier S & T
  • SKU 9780126536300
  • ISBN-13 9780080550183
  • Author Snapka, Robert M.
  • Category Medical
  • Subject Biotechnology

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Description eBook delivery & access Reviews
This book provides the most up-to-date review of the simian virus 40 (SV40) minichromosome as a model for the mammalian chromosome in studies of DNA replication. It focuses on disruption of DNA replication by anticancer drugs and DNA-damaging agents. There is a strong emphasis on the unique advantages of SV40 as an experimental system for the analysis of these classes of anticancer drug mechanisms. The new high-resolution gel electrophoresis methods for the analysis of SV40 DNA replication are covered in detail to aid readers in designing and interpreting similar experiments.

Key Features
* Presents unique advantages of SV40 as an experimental system for the study of classes of anticancer drugs
* Details new high-resolution gel electrophoresis methods for the analysis of SV40 DNA replication
* Provides details to help the reader design and interpret similar experiments