Elsevier S & T

The Zebrafish: Cellular and Developmental Biology: Cellular and Developmental Biology

Detrich, III, H. William

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Table of contents

Cover
Contentsv
Contributorsxiii
Prefacexix
Dedicationxxi
Part I: Cell Biology1
Chapter 1. Use of the DsRed Fluorescent Reporter in Zebrafish3
I. Introduction3
II. DsRed: History and Properties4
III. DsRed in Transgenic Zebrafish5
IV. Use of Multiple Fluorescent Reporters in Transplantation Assays7
V. Fusion Protein Reporters10
VI. Conclusion11
References11
Chapter 2. Analyzing Axon Guidance in the Zebrafish Retinotectal System13
I. Introduction13
II. Retinotectal Mutants14
III. Labeling the Retinotectal System16
IV. Perturbing the Retinotectal System27
V. Future Directions31
References32
Chapter 3. Optical Projection Tomography for Spatio-Temporal Analysis in the Zebrafish37
I. Introduction38
II. The Principle of Tomography39
III. Problems for OPT in Zebrafish41
IV. Materials42
V. Methods42
VI. Sample Preparation43
VII. Scanning44
VIII. Reconstruction45
IX. Presentation of Reconstructions46
X. Discussion49
XI. The Future of OPT49
XII. OPT Equipment50
References50
Chapter 4. Imaging Blood Vessels in the Zebrafish51
I. Introduction51
II. Imaging Vascular Gene Expression53
III. Nonvital Blood Vessel Imaging54
IV. Vital Imaging of Blood Vessels63
V. Conclusion73
References73
Chapter 5. Zebrafish Apoptosis Assays for Drug Discovery75
I. Introduction75
II. Zebrafish Apoptosis Genes76
III. Caspase Substrate Recognition in Zebrafish77
IV. Detection of Apoptosis In Situ77
V. Perspectives82
References83
Chapter 6. Lipid Metabolism in Zebrafish87
I. Introduction87
II. Genetic Screen„Forward, Reverse, and Targeted-Mutagenesis88
III. Pharmacological Studies Using Zebrafish93
IV. Mechanism of Action Studies Using Zebrafish. The Annexin2-Caveolin1 Complex99
V. Gene-Specific Studies: The Acyl-CoA Synthetase Enzymes102
VI. Summary104
References104
Chapter 7. Analysis of the Cell Cycle in Zebrafish Embryos109
I. Introduction: The Cell Cycle in Zebrafish109
II. Zebrafish Embryo Cell Cycle Protocols111
III. Screening for Mutations Affecting the Zebrafish Cell Cycle118
IV. Screening for Chemical Suppressors of Zebrafish Cell Cycle Mutants119
V. Conclusions122
VI. Reagents and Supplies123
References123
Chapter 8. Cellular Dissection of Zebrafish Hematopoiesis127
I. Introduction127
II. Zebrafish Hematopoiesis128
III. Hematopoietic Cell Transplantation136
IV. Enrichment of Hematopoietic Stem Cells144
V. Conclusions147
References147
Chapter 9. Culture of Embryonic Stem Cell Lines from Zebrafish151
I. Introduction151
II. Methods152
III. Materials158
References160
Part II: Developmental and Neural Biology161
Chapter 10. Neurogenesis163
I. Introduction164
II. The Primary Neuronal Scaffold164
III. Early Development of the Zebrafish Neural Plate167
IV. Lateral Inhibition and the Neurogenesis Cascade174
V. Establishment of Neuronal Identity176
VI. Aspects of Neuronal Differentiation179
VII. Useful Tools for the Study of Zebrafish Neurogenesis181
VIII. Conclusion190
References190
Chapter 11. Time-Lapse Microscopy of Brain Development207
I. Introduction„Why and When to Use Intravital Imaging208
II. Techniques for Vital Staining of the Nervous System209
III. Preparation of the Zebrafish Specimen218
IV. The Microscopic System221
V. Data Recording222
VI. Data Analysis225
VII. Pitfalls to Avoid229
VIII. Summary231
References232
Further Reading235
Chapter 12. Development of the Peripheral Sympathetic Nervous System in Zebrafish237
I. Introduction237
II. The Peripheral Autonomic Nervous System238
III. The Zebrafish as a Model System for Studying PSNS Development242
IV. Zebrafish as a Novel Model for Studying Neuroblastoma254
V. Conclusion and Future Directions255
References256
Chapter 13. Optical Physiology and Locomotor Behaviors of Wild-Type and Nacre Zebrafish261
I. Introduction262
II. Optical Physiology„Basics263
III. Optical Physiology„Alternative Approaches267
IV. Options for Improving the Transparency of Larval Zebrafish272
V. Behavioral Perturbation Studies276
VI. Conclusions280
References280
Chapter 14. Computer-Aided Screening for Zebrafish Embryonic Motility Mutants285
I. Introduction286
II. Flatbed Transparency Scanners and Computers288
III. Software290
IV. Embryos and Larva: Physiological Considerations291
V. Data Acquisition294
VI. Data Analysis294
VII. Potential Problems and Remedies305
VIII. Conclusions311
References312
Chapter 15. Photoreceptor Structure and Development: Analyses Using GFP Transgenes315
I. Introduction316
II. Photoreceptor Development318
III. Transgenic Zebrafish Expressing Photoreceptor-Specific Reporter Genes320
IV. Developmental Studies of Photoreceptors Using Transgenic Zebrafish322
V. Conclusions327
References328
Chapter 16. Approaches to Study Neurogenesis in the Zebrafish Retina333
I. Introduction333
II. Development of the Zebrafish Retina335
III. Analysis of Wild-Type and Mutant Zebrafish Visual Systems341
IV. Genetic Analysis of the Zebrafish Retina359
V. Summary374
References375
Chapter 17. Instrumentation for Measuring Oculomotor Performance and Plasticity in Larval Organisms385
I. Introduction386
II. Methods389
III. Results and Discussion395
IV. Conclusion407
V. Supplemental Movie Descriptions407
References411
Chapter 18. Development of Cartilage and Bone415
I. Introduction415
II. Cartilage Visualization Techniques419
III. Bone Visualization Techniques427
IV. Molecular Markers of Skeletal Precursors431
V. Strategy and Potential of Future Screens for Skeletal Mutants431
References433
Chapter 19. Morphogenesis of the Jaw: Development Beyond the Embryo437
I. Larval Zebrafish Craniofacial Cartilage Development437
II. Analysis of Craniofacial Skeletal and Replacement Tooth Development439
III. Conclusion451
References451
Chapter 20. Cardiac Development455
I. Introduction455
II. Stages of Heart Tube Morphogenesis456
III. Gene Expression465
IV. Conclusion and Future Directions469
References470
Chapter 21. Chemical Approaches to Angiogenesis475
I. Introduction475
II. Screening Tools479
III. Chemical Screening482
IV. Sensitized Screens485
References485
Chapter 22. Vascular Occlusion and Thrombosis in Zebrafish489
I. Introduction489
II. Vascular Occlusion491
III. Methods493
IV. Future Perspectives496
References498
Chapter 23. Zebrafish Kidney Development501
I. Introduction501
II. Pronephric Structure and Function502
III. Pronephric Development504
IV. The Zebrafish Pronephros as a Model of Human Disease521
V. Conclusions524
References525
Chapter 24. Pancreas Development in Zebrafish531
I. Pancreas Organogenesis531
II. Morphology of the Mature Pancreas532
III. Physiology of the Endocrine Pancreatic Tissues533
IV. Pancreas Development533
V. Formation and Patterning of the Zebrafish Endoderm533
VI. Formation of the Pancreatic Primordium537
VII. Cell Type Specification and Differentiation of the Endocrine Pancreas542
VIII. Formation of the Exocrine Pancreas544
IX. Perspectives546
References547
Part III: Disease Models553
Chapter 25. Mosaic Eyes, Genomic Instability Mutants, and Cancer Susceptibility555
I. Introduction556
II. Mutagenesis and Screening for Mosaic Eyes561
III. Mosaic Eyes. Genomic Instability or Retinal Mutations?561
IV. Studying Cancer Susceptibility in Adult Zebrafish562
V. Transgenic Analysis of Genomic Instability564
VI. Summary566
References566
Chapter 26. Discovery and Use of Small Molecules for Probing Biological Processes in Zebrafish569
I. Rationale for Small Molecule Screens in Zebrafish570
II. Assay Development575
III. Chemical Suppressor and Enhancer Screens577
IV. Selection of Small Molecule Libraries579
V. Screening Methods582
VI. Follow-Up Studies for Active Small Molecules584
VII. Conclusions588
References588
Chapter 27. Modeling Human Disease by Gene Targeting593
I. Introduction594
II. DNA Repair Processes595
III. Transient Means to Effect Altered Gene Expression in the Zebrafish600
IV. Heritable Means to Effect Altered Gene Expression in the Zebrafish607
V. Discussion608
References609
Index613
Volumes in Series625

Book details

Vendor Elsevier S & T
SKU 9780125641715
ISBN-13 9780080473451
Author Detrich, III, H. William
Edition 2nd
Category Science
Subject Cell Biology

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This volume of Methods in Cell Biology, the first of two parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 1999. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases.

* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
* Illustrates many techiques in full-color
* Summarizes the Zebrafish Genome Project

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