Vertebrae Phototransduction and the Visual Cycle, Part B
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Table of contents
- Table of Contentsv
- Contributors to Volume 316xi
- Prefacexvii
- Volumes in Seriesxix
- Section I: Photoreceptor Proteins1
- Chapter 1. Inhibition of Rhodopsin Phosphorylation by S- Modulins: Purification, Reconstitution, and3
- Chapter 2. Analysis of Protein–Protein Interactions in Phototransduction Cascade Using Surface Pla20
- Chapter 3. Expression of Phototransduction Proteins in Xenopus Oocytes41
- Chapter 4. Xenopus Rod Photoreceptor: Model for Expression of Retinal Genes50
- Chapter 5. Fusion between Retinal Rod Outer Segment Membranes and Model Membranes: Functional Assays65
- Chapter 6. Bovine Retinal Nucleoside Diphosphate Kinase: Biochemistry and Molecular Cloning87
- Section II: Calcium-Binding Proteins and Calcium Measurements in Photoreceptor Cells101
- Chapter 7. Calcium-Binding Proteins and Their Assessment in Ocular Diseases103
- Chapter 8. Molecular Structure of Membrane-Targeting Calcium Sensors in Vision: Recoverin and Guanyl121
- Chapter 9. Measurement of Light-Evoked Changes in Cytoplasmic Calcium in Functionally Intact Isolate133
- Chapter 10. Laser Spot Confocal Technique to Measure Cytoplasmic Calcium Concentration in Photorecep146
- Section III: Phototransduction165
- Chapter 11. Functional Study of Rhodopsin Phosphorylation in Vivo167
- Chapter 12. Mechanisms of Single-Photon Detection in Rod Photoreceptors186
- Chapter 13. Electroretinographic Determination of Human Rod Flash Response in Vivo202
- Chapter 14. Electrophysiological Methods for Measurement of Activation of Phototransduction by Bleac224
- Chapter 15. Exploring Kinetics of Visual Transduction with Time-Resolved Microcalorimetry253
- Chapter 16. Use of a-Toxin-Permeabilized Photoreceptors in in Vitro Phototransduction Studies269
- Chapter 17. Photoreceptors in Pineal Gland and Brain: Cloning, Localization, and Overexpression278
- Chapter 18. Cultured Amphibian Melanophores: A Model System to Study Melanopsin Photobiology291
- Section IV: Enzymes of the Visual Cycle311
- Chapter 19. High-Performance Liquid Chromatography Analysis of Visual Cycle Retinoids313
- Chapter 20. Quantitative Measurements of Isomerohydrolase Activity324
- Chapter 21. Multienzyme Analysis of Visual Cycle330
- Chapter 22. Analyzing Membrane Topology of 11-cis-Retinol Dehydrogenase344
- Chapter 23. Phase Partition and High-Performance Liquid Chromatography Assays of Retinoid Dehydrogen359
- Chapter 24. Short-Chain Dehydrogenases/Reductases in Retina372
- Chapter 25. Substrate Specificities of Retinyl Ester Hydrolases in Retinal Pigment Epithelium384
- Chapter 26. Molecular Characterization of Lecithin-Retinol Acyltransferase400
- Chapter 27. Analysis of Chromophore of RGR: Retinal G-Protein–Coupled Receptor from Pigment Epithe413
- Section V: Posttranslational and Chemical Modifications423
- Chapter 28. Low Temperature Photoaffinity Labeling of Rhodopsin and Intermediates along Transduction425
- Chapter 29. Structural Analysis of Protein Prenyl Groups and Associated C-Terminal Modifications436
- Chapter 30. Isoprenylation/Methylation and Transducin Function451
- Chapter 31. Functional Analysis of Farnesylation and Methylation of Transducin465
- Chapter 32. Identification of Phosphorylation Sites within Vertebrate and Invertebrate Rhodopsin482
- Chapter 33. Ocular Proteomics: Cataloging Photoreceptor Proteins by Two-Dimensional Gel Electrophore492
- Section VI: Analysis of Animal Models of Retinal Diseases513
- Chapter 34. Animal Models for Inherited Retinal Diseases515
- Chapter 35. Avian Models of Inherited Retinal Disease526
- Chapter 36. Biochemical Analysis of Phototransduction and Visual Cycle in Zebrafish Larvae536
- Chapter 37. Genetic Models to Study Guanylyl Cyclase Function558
- Chapter 38. Analysis of Visual Cycle in Normal and Transgenic Mice565
- Section VII: Inherited Retinal Disease: From the Defective Gene to Its Function and Repair577
- Chapter 39. In Situ Hybridization Studies of Retinal Neurons579
- Chapter 40. Cloning and Characterization of Retinal Transcription Factors, Using Target Site–Based590
- Chapter 41. In Vivo Assessment of Photoreceptor Function in Human Diseases Caused by Photoreceptor-S611
- Chapter 42. Spectral Sensitivities of Human Cone Visual Pigments Determined in Vivo and in Vitro626
- Chapter 43. Molecular Analysis of Human Red/Green Visual Pigment Gene Locus: Relationship to Color V651
- Chapter 44. Expression and Characterization of Peripherin/rds rom-1 Complexes and Mutants Implicated671
- Chapter 45. Isolation of Retinal Proteins That Interact with Retinitis Pigmentosa GTPase Regulator b688
- Chapter 46. Genetic Analysis of RPE65: From Human Disease to Mouse Model705
- Chapter 47. Construction of Encapsidated Gutted Adenovirus Minichromosomes and Their Application to724
- Chapter 48. Production and Purification of Recombinant Adeno-Associated Virus743
- Chapter 49. Ribozymes in Treatment of Inherited Retinal Disease761
- Chapter 50. Cross-Species Comparison of in Vivo Reporter Gene Expression after Recombinant Adeno-Ass777
- Author Index791
- Subject Index827
Major topics covered include photoreceptor proteins, phototransduction calcium-binding proteins and calcium measurement in photoreceptor cells, enzymes of the visual cycle, posttranslational and chemical modifications, analysis of animal models of retinal diseases. Inherited retinal disease; from the defective gene to its function and repair. This volume and its companion Volume 315 include newly developed methods to study vertebrate phototransduction and the visual cycle.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.