Elsevier S & T

Vertebrate Phototransduction and the Visual Cycle, Part A

Abelson, John N.

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Table of contents

Table of Contentsv
Prefacexvii
Volumes in Seriesxix
Section I: Expression and Isolation of Opsins1
Chapter 1. Heterologous Expression of Bovine Opsin in Pichia pastoris3
Chapter 2. Baculovirus Expression System for Expression and Characterization of Functional Recombina12
Chapter 3. Development of Stable Cell Lines Expressing High Levels of Point Mutants of Human Opsin f30
Chapter 4. Folding and Assembly of Rhodopsin from Expressed Fragments59
Chapter 5. Isolation of Isoelectric Species of Phosphorylated Rhodopsin70
Chapter 6. Rhodopsin Trafficking in Photoreceptors Using Retinal Cell-Free System77
Section II: Characterization of Opsins89
Chapter 7. Preparation and Analysis of Two-Dimensional Crystals of Rhodopsin91
Chapter 8. Domain Approach to Three-Dimensional Structure of Rhodopsin Using High Resolution Nuclear107
Chapter 9. Analysis of Functional Microdomains in Rhodopsin116
Chapter 10. Mapping Tertiary Contacts between Amino Acid Residues within Rhodopsin130
Chapter 11. Energetics of Rhodopsin Photobleaching: Photocalorimetric Studies of Energy Storage in E143
Chapter 12. Absorption Spectroscopy in Studies of Visual Pigments. Spectral and Kinetic Characteriza164
Chapter 13. Structural Determinants of Active State Conformation of Rhodopsin. Molecular Biophysics178
Chapter 14. pKa of Protonated Schiff Base of Visual Pigments196
Chapter 15. Assays for Detection of Constitutively Active Opsins207
Chapter 16. Synthetic Retinals: Convenient Probes of Rhodopsin and Visual Transduction Process219
Chapter 17. Assays for Activation of Opsin by all-trans-Retinal238
Chapter 18. Assays for Activation of Recombinant Expressed Opsin by all-trans-Retinal251
Chapter 19. Electrical Approach to Study Rhodopsin Activation in Single Cells with Early Receptor Cu268
Chapter 20. Analysis of Amino Acid Residues in Rhodopsin and Cone Visual Pigments That Determine The293
Chapter 21. Phylogenetic Analysis and Experimental Approaches to Study Color Vision in Vertebrates312
Section III: Proteins That Interact with Rhodopsin327
Chapter 22. Light Scattering Methods to Monitor Interaction between Rhodopsin-Containing Membranes a329
Chapter 23. Heterogeneity of Rhodopsin Intermediate State Interacting with Transducin347
Chapter 24. Limited Proteolytic Digestion Studies of G Protein Receptor Interaction363
Chapter 25. Monitoring Proton Uptake from Aqueous Phase during Rhodopsin Activation377
Chapter 26. Use of Peptides-on-Plasmids Combinatorial Library to Identify High Affinity Peptides Tha388
Chapter 27. Purifiation of Rhodopsin Kinase by Recoverin Affinity Chromatography404
Chapter 28. Heterologous Expression and Reconstitution of Rhodopsin with Rhodopsin Kinase and Arrest411
Chapter 29. Arrestin: Mutagenesis, Expression, Purification, and Functional Characterization422
Chapter 30. Mapping Interaction Sites between Rhodopsin and Arrestin by Phage Display and Synthetic437
Chapter 31. Characterization of RanBP2-Associated Molecular Components in Neuroretina455
Section IV: Transducin and Regulators of G-Protein Signaling469
Chapter 32. Intrinsic Biophysical Monitors of Transducin Activation. Fluorescence, UV/Visible Spectr471
Chapter 33. Fluorescent Probes as Indicators of Conformation Changes in Transducin on Activation490
Chapter 34. Gat/Gai1 Chimeras Used to Define Structural Basis of Specific Functions of Gat502
Chapter 35. Enzymology of GTPase Acceleration in Phototransduction524
Chapter 36. Mutational Analysis of Effectors and Regulators of G-Protein Signaling Interfaces of Tra539
Section V: Photoreceptor Protein Phosphatases555
Chapter 37. Isolation and Properties of Protein Phosphatase 2A in Photoreceptors557
Chapter 38. Purifiation and Characterization of Protein Phos- phate Type 2C in Photoreceptors570
Chapter 39. Photoreceptor Serine/Threonine Protein Phosphatase Type 7: Cloning, Expression, and Func579
Section VI: Photoreceptor Phosphodiesterase and Guanylyl Cyclase595
Chapter 40. Purification and Assay of Bovine Type 6 Photoreceptor Phosphodiesterase and Its Subunits597
Chapter 41. Transcriptional Regulation of cGMP Phosphodiesterase B-Subunit Gene617
Chapter 42. Inhibition of Photoreceptor cGMP Phosphodiesterase by Its y Subunit635
Chapter 43. Kinetics and Regulation of cGMP Binding to Non- catalytic Binding Sites on Photoreceptor646
Chapter 44. Purifiation and Autophosphorylation of Retinal Guanylate Cyclase673
Chapter 45. Use of Nucleotide a-Phosphorothioates in Studies of Photoreceptor Guanylyl Cyclase: Puri689
Chapter 46. Heterologous Expression and Assays for Photoreceptor Guanylyl Cyclases and Guanylyl Cycl708
Chapter 47. Spectrophotometric Determination of Retinal Rod Guanylyl Cyclase718
Chapter 48. Calcium-Dependent Activation of Membrane Guanylate Cyclase by S100 Proteins730
Chapter 49. Characterization of Guanylyl Cyclase and Phosphodiesterase Activities in Single Rod Oute742
Section VII: Cyclic Nucleotide-Gated Channels753
Chapter 50. Covalent Tethering of Ligands to Retinal Rod Cyclic Nucleotide-Gated Channels: Binding S755
Chapter 51. Using State-Specific Modifiers to Study Rod cGMP- Activated Ion Channels Expressed in Xe772
Chapter 52. Identification and Characterization of Calmodulin Binding Sites in cGMP-Gated Channel Us785
Chapter 53. Determination of Fractional Calcium Ion Current in Cyclic Nucleotide-Gated Channels797
Chapter 54. Modulation of Rod cGMP-Gated Cation Channel by Calmodulin817
Section VIII: Na+ /Ca2+ -K+ Exchanger and ABCR Transporter829
Chapter 55. Purification and Biochemical Analysis of cGMP- Gated Channel and Na+ /Ca2+ -K+ Exchange831
Chapter 56. Spectrofiuorometric Detection of Na+ /Ca2+ -K+ Exchange847
Chapter 57. Purification and Characterization of ABCR from Bovine Rod Outer Segments864
Chapter 58. ABCR: Rod Photoreceptor-Specific ABC Transporter Responsible for Stargardt Disease879
Author Index899
Subject Index935

Book details

Vendor Elsevier S & T
SKU 9780121822163
ISBN-13 9780080496726
Author Abelson, John N.
Category Science
Subject Biochemistry

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This volume, and its companion Volume 316, include newly developed methods to study vertebrate phototransduction and the visual cycle. Major topics covered include expression, isolation, and characterization of opsins; proteins that interact with rhodopsin; transducin and regulators of G-protein signaling; photoreceptor protein phosphatases, phosphodiesterase, and guanylylcyclase; cyclis nucleolide gated channels; Na+/Ca2+-K+ exchanges and ABCR transporter.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

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