Viral Gene Techniques: Viral Gene Techniques
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Table of contents
- Table of Contentsv
- Contributors to Volume 7ix
- Prefacexv
- Volumes in Seriesxvii
- Section I: Viral Vectors1
- Chapter 1. Adeno-Associated Virus: Integrating Vectors for Human Gene Therapy3
- Chapter 2. Techniques for Human Adenovirus Vector Construction and Characterization13
- Chapter 3. Construction and Isolation of Recombinant Adenoviruses with Gene Replacements31
- Chapter 4. Construction and Use of Recombinant Vaccinia Virus Vectors45
- Chapter 5. Epstein–Barr Viral Plasmid Vectors and Their Amplifiable Derivatives65
- Section II: DNA Viruses87
- Chapter 6. Methods to Study Epstein–Barr Virus and p53 Status in Human Cells89
- Chapter 7. The SV40 Minichromosome101
- Chapter 8. Site-Specific Integration of Multigenic Shuttle Plasmids into the Herpes Simplex Virus Ty114
- Chapter 9. Strategies for Studying Transcription Regulation of Neurotropic Viruses in the Central Ne131
- Chapter 10. Analysis of the Nuclear DNA-Binding Activity in Cells Overexpressing Hepatitis B Viral X152
- Chapter 11. Investigations on Virus-Host Interactions: An Abortive System167
- Section III: Human Immunodeficiency Virus/Retroviruses193
- Chapter 12. Quantitation of Virus Stocks Produced from Cloned Human Immunodeficiency Virus DNA195
- Chapter 13. Quantitation of HIV-1 RNA in Plasma or Serum Samples209
- Chapter 14. Detection of Cell Fusion Mediated by the Envelopes of Human Retroviruses by Transactivat218
- Chapter 15. Assembly of Recombinant Retroviral Gag Precursors into Pseudovirions in the Baculovirus237
- Chapter 16. Retrovirus Reverse Transcription and Integration254
- Chapter 17. In Vitro Binding and Transcription Assays Using the Human T-Cell Leukemia Virus Type I T267
- Chapter 18. Polymerase Chain Reaction-Based Techniques for Utilizing Retroviruses as Cell Lineage Ma280
- Section IV: RNA Viruses297
- Chapter 19. New Methods to Study Poliovirus Assembly and Encapsidation of Genomic RNA299
- Chapter 20. Molecular Studies of Hepatitis Delta Virus RNAs315
- Chapter 21. Systems to Express Recombinant RNA Molecules by the Influenza A Virus Polymerase in Vivo329
- Chapter 22. Analysis of Membrane Association in Vivo and Targeting of Viral Proteins in Polarized Ep343
- Chapter 23. Cloning of Viral Double-Stranded RNA Genomes by Single Primer Amplification359
- Chapter 24. Using the RNA-Capture Assay to Assess the RNA-Binding Activity of Viral Proteins373
- Chapter 25. Protein RNA-Binding Activity Measured by Northwestern Blot Analysis: The Interferon-Indu388
- Erratum404
- Index405
Viral Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The volume is concerned with methods for the analysis of viral genes and chromosomes: DNA viruses and RNA viruses including HIV are discussed.
* Methods presented for ease of use and reasdy adaptation to new systems
* Detailed experimental protocols included for:
* Viral vectors - construction and use of DNA virus vectors (adenovirus, adeno-associated virus, vaccinia virus, Epstein - Barr virus)
* DNA viruses - virus/host interactions, viral chromosomes , transcription regulation (viruses discussed include herpes simplex, hepatitis B, SV40, JC, Epstein-Barr, adenovirus)
* Human Immunodeficiency Virus / retroviruses -quantitation of HIV-1 virus stock and RNA, retrovirus reverse transcription / integration, retrovirus-mediated cell fusion, use as cell lineage markers
* RNA viruses - RNA virus assembly, analysis of RNA genomes, assays for RNA-binding proteins (viruses discussed include poliovirus, influenza virus, hepatitis delta virus)
* Methods presented for ease of use and reasdy adaptation to new systems
* Detailed experimental protocols included for:
* Viral vectors - construction and use of DNA virus vectors (adenovirus, adeno-associated virus, vaccinia virus, Epstein - Barr virus)
* DNA viruses - virus/host interactions, viral chromosomes , transcription regulation (viruses discussed include herpes simplex, hepatitis B, SV40, JC, Epstein-Barr, adenovirus)
* Human Immunodeficiency Virus / retroviruses -quantitation of HIV-1 virus stock and RNA, retrovirus reverse transcription / integration, retrovirus-mediated cell fusion, use as cell lineage markers
* RNA viruses - RNA virus assembly, analysis of RNA genomes, assays for RNA-binding proteins (viruses discussed include poliovirus, influenza virus, hepatitis delta virus)