Elsevier S & T

Virology Methods Manual

Kangro, Hillar O.; Mahy, Brian W.J.

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Table of contents
  • Cover
  • Contentsvii
  • Contributorsix
  • SECTION 1: Classical techniques1
  • Chapter 1. Cell Culture3
  • Chapter 2. Virus Isolation and Quantitation24
  • Chapter 3. Preparation of Antigens47
  • Chapter 4. Virus Purification71
  • Chapter 5. Electron Microscopy91
  • Chapter 6. Traditional Serological Tests107
  • Chapter 7. Immunoassays: Principles and Assay Design123
  • Chapter 8. Cell Mediated Immunity143
  • SECTION 2: Techniques in molecular virology163
  • Chapter 9. RNA: Transcription, Transfection and Quantitation165
  • Chapter 10. DNA Viruses: DNA Extraction, Purification and Characterization191
  • Chapter 11. Virus Mutants231
  • Chapter 12. Polypeptides251
  • Chapter 13. The Assay of Viral Enzymes277
  • SECTION 3: Medical Virology291
  • Chapter 14. Initial in vitro Screening of Drug Candidates for their Potential Antiviral Activities293
  • Chapter 15. Detection of Virus and Viral Antigens309
  • Chapter 16. Detection of Viral Nucleic Acids in Clinical Material323
  • Chapter 17. Serological Diagnosis343
  • Chapter 18. Disinfection353
  • APPENDICES357
  • A. Biosafety in the Virology Laboratory357
  • B. Metabolic Inhibitors Used in Virology363
  • C. Virus Taxonomy365
  • Index367
Book details
  • Vendor Elsevier S & T
  • SKU 9780124653306
  • ISBN-13 9780080543581
  • Author Kangro, Hillar O.; Mahy, Brian W.J.
  • Category Medical
  • Subject Diseases

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The Virology Methods Manual is a comprehensive source of methods for the study, manipulation, and detection of viruses. Edited by Brian Mahy and Hillar Kangro, this work describes the most up-to-date, definitive techniques, provided by experts in each area, and presented with easy-to-use, step-by-step protocols. This new manual will satisfy the needs of virologists and all those working with viruses who need a practical guide to methods that work!

Key Features
* Provides up-to-date techniques by experts worldwide
* Presents common, step-by-step protocols in an attractive, easy-to-use fashion
* Contains useful appendices including virus taxonomy, metabolic inhibitors, and Bio-safety in the virology laboratory